Coding
Part:BBa_K5097015:Experience
Designed by: Christopher Dorce Group: iGEM24_Oneonta (2024-09-25)
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Applications of BBa_K5097015
Team Oneonta 2024 used this part to purify RFP for studies on the effect of pH on the emission of this fluorescent protein. We expressed composite parts BBa_K5097016 and BBa_K5097017 in E. coli DH5alpha cells, and lysed the cells by treating with lysozyme and sonication. Proteins were purified via Ni-IMAC chromatography and eluted in 50mM sodium phosphate, 300mM sodium chloride, and 500mM imidazole at pH8.0. Proteins were concentrated and exchanged into PBS via centrifugal diafiltration.
- Figure 1: Coomasie stained PAGE gel showing purification of BFP-6XHis and RFP-6XHis
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